Maximal culture density

Range 1.6-20.5 g/L
Organism Various
Reference Puskeiler R, Kaufmann K, Weuster-Botz D. Development, parallelization, and automation of a gas-inducing milliliter-scale bioreactor for high-throughput bioprocess design (HTBD). Biotechnol Bioeng. 2005 Mar 589(5):512-23. Table - link PubMed ID15669089
Primary Source See table for references
Method Luria–Bertani (LB) medium, comprised of 10 g/L yeast extract, 5 g/L NaCl, and 5 g/L peptone, was used for sterile tests. Automated Fed-Batch Cultivation at Milliliter Scale (5 mL). Cell growth was monitored atline in MTPs (microtiter plates) by measuring the OD at a wavelength of 650 nm. After unlimited batch growth, a linear feeding profile was started. The culture in the bioreactor with the standard magnetic bar showed less biomass formation during the course of the cultivation. During batch growth, the culture suffered from oxygen limitation as indicated by the increased drop in pH per hour, which was visible because titration was carried out intermittently at a frequency of 1/h. During fed batch, the culture in the reactor equipped with the gas-inducing impeller reached a DCW of 20.5 g/L (see table link).
Comments The classic parallel-operated bioreactor for process development is the shake flask (Bu¨chs, 2001a). Up to 16 parallel flasks can be run with pH control in fed-batch mode (Weuster-Botz et al., 2001a), but further parallelization on the basis of this technology seems difficult. Far more parallel experiments can be performed in shaken microtiter plates (MTPs) (Duetz et al., 2000 John et al., 2003 Minas et al., 2000).
Entered by Uri M
ID 104940