| Range |
1.6-20.5 g/L
|
| Organism |
Various |
| Reference |
Puskeiler R, Kaufmann K, Weuster-Botz D. Development, parallelization, and automation of a gas-inducing milliliter-scale bioreactor for high-throughput bioprocess design (HTBD). Biotechnol Bioeng. 2005 Mar 589(5):512-23. Table - link PubMed ID15669089
|
| Primary Source |
See table for references |
| Method |
Luria–Bertani (LB) medium, comprised of 10 g/L yeast
extract, 5 g/L NaCl, and 5 g/L peptone, was used for
sterile tests. Automated Fed-Batch Cultivation at Milliliter
Scale (5 mL). Cell growth was monitored atline in MTPs (microtiter plates) by measuring
the OD at a wavelength of 650 nm. After unlimited batch growth, a linear feeding profile was started. The culture in the bioreactor with the standard
magnetic bar showed less biomass formation during the course of the cultivation. During batch growth, the culture suffered from oxygen limitation as indicated by the increased drop in pH per hour, which was visible because titration was carried out intermittently at a frequency
of 1/h. During fed batch, the culture in the reactor equipped with the gas-inducing impeller reached a
DCW of 20.5 g/L (see table link). |
| Comments |
The classic parallel-operated bioreactor for process development
is the shake flask (Bu¨chs, 2001a). Up to 16
parallel flasks can be run with pH control in fed-batch
mode (Weuster-Botz et al., 2001a), but further parallelization
on the basis of this technology seems difficult. Far
more parallel experiments can be performed in shaken
microtiter plates (MTPs) (Duetz et al., 2000 John et al.,
2003 Minas et al., 2000). |
| Entered by |
Uri M |
| ID |
104940 |