RNA polymerase transcription rate of mRNA (at 37°C)

Range 39 - 55 Table - link nucleotides/sec
Organism Bacteria Escherichia coli
Reference Bremer, H., Dennis, P. P. (1996) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed. chapter 97 Table 3
Primary Source [19] Bremer H, Yuan D. RNA chain growth-rate in Escherichia coli. J Mol Biol. 1968 Dec 14 38(2):163-80. [139] Vogel U, Jensen KF. The RNA chain elongation rate in Escherichia coli depends on the growth rate. J Bacteriol. 1994 May176(10):2807-13PubMed ID4941474, 7514589
Method Footnote 'g' beneath table:"The mRNA chain elongation rate was determined by analysis of pulse-labeling kinetics of RNA after size fractionation [primary source 19] and by the time lag between induction of transcription of specific mRNAs (lacZ, infB) and the appearance of specific hybridization to DNA probes from the 3' ends of the respective genes [primary source 139]."
Comments Both primary sources at 37°C.
Entered by Uri M
ID 104900