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Metal ion activation of partially purified corn alkaline inorganic pyrophosphatase

Range	Table - link
Organism	Corn Zea mays
Reference	J. W. RIP and W. E. RAUSER, PARTIAL PURIFICATION AND SOME PROPERTIES OF ALKALINE INORGANIC PYROPHOSPHATASE , Phytochemistry1, 971, Vol. 10, pp. 2615 to 2619 Table 1
Method	The metals were used as their chlorides in the standard assay lacking Mg2+. The enzyme preparation used was that following DEAE-cellulose column chromatography and dialysis against 1 mM mercaptoethanol, 50 mM Tris-HCl (pH 8.7). A volume equivalent to 0.26 units of pyrophosphatase activity prior to dialysis was added to each reaction tube.
Entered by	Uri M
ID	104836