Phospholipid composition of several E. coli strains

Range Table - link
Organism Bacteria Escherichia coli
Reference Ganong BR, Raetz CR. Massive accumulation of phosphatidic acid in conditionally lethal CDP-diglyceride synthetase mutants and cytidine auxotrophs of Escherichia coli. J Biol Chem. 1982 Jan 10 257(1):389-94PubMed ID6273438
Method When 100 micrograms/ml erythromycin is added to nutrient agar plates, it is possible to obtain a 30-fold enrichment for cds mutants from a mutagen-treated stock, as judged by colony autoradiography. Using this approach researchers isolated 38 new cds mutants, nine of which are unable to grow at a culture pH greater than 8
Comments The quantitative phospholipid compositions of GN85 (cds+), GN80 (cds-8), and GN80-8 (a spontaneous pH-resistant revertant of GN80) were determined in samples taken immediately before and about 2 h after the pH shift (Table link). The compositions of GN85 and GN80-8 were similar- neither accumulated significant amounts of phosphatidic acid, and both contained slightly more phosphatidylglycerol at pH 8.5.
Entered by Uri M
ID 104809