Table - link
||Tree Norway Spruce Picea abies
||Albrechtová J, Janácek J, Lhotáková Z, Radochová B, Kubínová L. Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles. J Exp Bot. 200758(6):1451-61PubMed ID17322549
||Stereological methods. First, the optical disector method was applied, yielding the number of mesophyll cells in a needle, mean mesophyll cell volume, and the ratio of intercellular spaces per mesophyll cell volume. The optical disector (Gundersen, 1986) is based on focusing through the 3D probe within a thick section.
||The comparison of selected mesophyll parameters was conducted to evaluate the effect of simulated acid rain (SAR) on current Norway spruce needles. Although the needle length did not differ between treated and untreated needles, needle volume significantly decreased after SAR application due to the decrease of cross-sectional area of treated needles (Fig. 2). The volume density of mesophyll did not change significantly after the treatment, while the volume of intercellular spaces per mesophyll cell volume increased, which corresponded well to the decrease in the number of mesophyll cells per unit volume of the needle. Also the total number of mesophyll cells decreased in treated needles. No significant change in mean mesophyll cell volume was observed. The internal surface area of a needle decreased after the SAR application, but the internal needle surface area per unit needle volume increased