Cleavage rate of pyrophosphate into Pi

Value 5.7 nmole Pi/min/ug PPi
Organism Bacteria Escherichia coli
Reference Kent RB, Guterman SK. Pyrophosphate inhibition of rho ATPase: a mechanism of coupling to RNA polymerase activity. Proc Natl Acad Sci U S A. 1982 Jul 79(13):3992-6. Table 3PubMed ID6125940
Method Preparation of p and Assay of ATPase, [tritium]Poly(C) Binding Assay, Kinetic Measurements, Pyrophosphatase Assays, Protein Determination,
Comments Incubated 15 min in absence of proteins. Pi yield is expressed as nmol/min per volume comparable to that used for assays of rho transcription termination factor in E. coli and yeast pyrophosphatase (BNID 104788-90).
Entered by Uri M
ID 104791