Metabolite levels and enzyme activities in wild-type and starchless mutant under different photoperiod regimes

Range Table - link
Organism Thale cress Arabidopsis thaliana
Reference Gibon Y, Pyl ET, Sulpice R, Lunn JE, Höhne M, Günther M, Stitt M. Adjustment of growth, starch turnover, protein content and central metabolism to a decrease of the carbon supply when Arabidopsis is grown in very short photoperiods. Plant Cell Environ. 2009 Jul32(7):859-74PubMed ID19236606
Method Plant growth. Arabidopsis thaliana var Col-0 was grown in soil [GS 90 soil mixed with vermiculite in a ratio 2:1 (v/v)]. After germination the seedlings were grown for 1 week in a 16/8 h light (250 µE m-2 s-1, 20 °C)/dark (6 °C) regime, for 1 week in an 8 h light (160 µE m-2 s-1, 20 °C)/16 h dark (16 °C) regime, and then replanted with one seedling per pot and transferred for 1 week to growth cabinets with an 8/16 h light–dark cycle (160 µE m-2 s-1, 20 °C throughout the day/night cycle). The plants were then distributed into small growth cabinets with a 12, 8, 4, 3 or 2 h photoperiod (all with a light intensity of 160 µE m-2 s-1 and 20 °C throughout the day/night cycle). The plants were harvested 17 d later. Typically, at least five separate samples, each containing three rosettes, were harvested. Extraction and assay of enzymes. Extraction and assay of metabolites and protein content.
Comments Legend for table: ED=End of Day. EN=End of Night. XN=Extended Night. pgm=starchless mutant.
Entered by Uri M
ID 104720