| Range |
Table - link
|
| Organism |
Thale cress Arabidopsis thaliana |
| Reference |
Gibon Y, Pyl ET, Sulpice R, Lunn JE, Höhne M, Günther M, Stitt M. Adjustment of growth, starch turnover, protein content and central metabolism to a decrease of the carbon supply when Arabidopsis is grown in very short photoperiods. Plant Cell Environ. 2009 Jul32(7):859-74PubMed ID19236606
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| Method |
Plant growth. Arabidopsis thaliana var Col-0 was grown in soil [GS 90 soil mixed with vermiculite in a ratio 2:1 (v/v)]. After germination the seedlings were grown for 1 week in a 16/8 h light (250 µE m-2 s-1, 20 °C)/dark (6 °C) regime, for 1 week in an 8 h light (160 µE m-2 s-1, 20 °C)/16 h dark (16 °C) regime, and then replanted with one seedling per pot and transferred for 1 week to growth cabinets with an 8/16 h light–dark cycle (160 µE m-2 s-1, 20 °C throughout the day/night cycle). The plants were then distributed into small growth cabinets with a 12, 8, 4, 3 or 2 h photoperiod (all with a light intensity of 160 µE m-2 s-1 and 20 °C throughout the day/night cycle). The plants were harvested 17 d later. Typically, at least five separate samples, each containing three rosettes, were harvested. Extraction and assay of enzymes. Extraction and assay of metabolites and protein content. |
| Comments |
Legend for table: ED=End of Day. EN=End of Night. XN=Extended Night. pgm=starchless mutant. |
| Entered by |
Uri M |
| ID |
104720 |