Intracellular metabolite concentrations in glucose-fed, exponentially growing E. coli

Range Table - link
Organism Bacteria Escherichia coli
Reference Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9 p.594 table 1PubMed ID19561621
Method P.594 right column top paragraph: "[Researchers] quantified metabolites by Liquid chromatography-mass spectrometry (LC-MS/MS) using an isotope ratio-based approach (ref 8). As isotope-labeled standards for many metabolites are not available, they used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (ref 25). This enabled the use of commercially available unlabeled compounds as internal standards."
Comments Abstract: "The total observed intracellular metabolite pool was approximately 300 mM (BNID 104678). A small number of metabolites dominate the metabolome on a molar basis, with glutamate being the most abundant." P.595 left column bottom paragraph: "The absolute cellular concentrations of 103 metabolites were determined in glucose-fed cultures, and most were also determined in glycerol- and acetate-fed cultures (for glucose-grown cells only, see Table 1 for complete data, including error estimates, see Supplementary Table 3)." P.596 left column bottom paragraph: "[Researchers] used the absolute concentration of metabolites in glucose-grown cultures (Table 1) to assess the extent of saturation of enzymes by their substrates, by comparing the measured concentrations to Km values taken from the BRENDA database (Fig. 2 and Supplementary Table 7)."
Entered by Uri M
ID 104679