Identification of specific binding partners from complex lipid mixtures
Range | Table - link |
---|---|
Organism | Unspecified |
Reference | Tagore R et al, A global metabolite profiling approach to identify protein-metabolite interactions, J Am Chem Soc. 2008 Oct 29 130(43):14111-3PubMed ID18831549 |
Method | Researchers looked at the binding interactions of fatty acid binding protein 2 (FABP2) and StAR-related lipid transfer (START) domain containing 3 (StarD3). FABP2 is an intracellular intestinal fatty acid binding protein that has been linked to metabolic disorders and cardiovascular disease. Researchers expressed FABP2 as a Glutathione -S-Transferase (GST) fusion (FABP-GST) and confirmed the activity upon binding of OA using an ANS displacement assay (Supporting Information). |
Entered by | Uri M |
ID | 104595 |