Fraction of Cell wall out of total cell weight

Value ~30 %
Organism Budding yeast Saccharomyces cerevisiae
Reference Lesage G, Bussey H. Cell wall assembly in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 2006 Jun70(2):317-43. p.318 left column 3rd paragraphPubMed ID16760306
Primary Source [206] Nguyen TH, Fleet GH, Rogers PL. Composition of the cell walls of several yeast species. Appl Microbiol Biotechnol. 1998 Aug50(2):206-12.PubMed ID9763691
Method (Primary source abstract:) "Cell walls, representing 26%-32% of the cell dry weight, were prepared from several strains of the yeasts Kloeckera apiculata, Debaryomyces hansenii, Zygosaccharomyces bailii, Kluyveromyces marxianus and Saccharomyces cerevisiae. Extraction of the walls with potassium hydroxide at 4 degrees C, followed by saturation of the alkali-soluble extract with ammonium sulphate gave fractions of mannoprotein, alkali-soluble glucan and alkali-insoluble glucan."
Comments P.318 left column 3rd paragraph: "The S. cerevisiae cell wall represents ~30% of the dry weight of the cell and is composed largely of polysaccharides (~85%) and proteins (~15%) (primary source)."
Entered by Uri M
ID 104588