7e-4 - 1e-3 gene/PFU
||Bacteria Escherichia coli
||Kenzaka T, Tani K, Sakotani A, Yamaguchi N, Nasu M. High-frequency phage-mediated gene transfer among Escherichia coli cells, determined at the single-cell level. Appl Environ Microbiol. 2007 May73(10):3291-9.PubMed ID17384307
||Purification of bacteriophages. Sequencing of isolated phage EC10. Plaque assays. Transduction and DNA transfer experiments. Viabilities of recipient cells determined by Direct Viable Counting (DVC). (cycling primed in situ amplification-fluorescent in situ hybridization [CPRINS-FISH]). Epifluorescence microscopy. Genetic characterization of transductants. PCR amplification of the unknown region next to the (beta lactamase gene-bla) bla sequence on transductants and sequencing. Examination of integration of bla gene into the phage genome.
||PFU=Plaque Forming Unit. Transduction frequencies determined by conventional plating were 3x10^(-8) - 2x10^(-6) gene/PFU