Diameter of endocytic vesicle

Value 100 nm Range: ±30 nm
Organism Mexican toad Bufo marinus
Reference Shi LB, Verkman AS. Very high water permeability in vasopressin-induced endocytic vesicles from toad urinary bladder. J Gen Physiol. 1989 Dec94(6):1101-15PubMed ID2515241
Method Mexican toads (Bufo marinus) were maintained on wet peat moss. Toads were killed by decapitation and excised urinary hemibladders were mounted on PE-190 polyethylene tubing (Becton Dickinson & Co., Parsippany, N J), serosa side out, using 3-0 silk suture. Endocytic vesicle morphology was studied by preparing the microsomal pellet using horseradish peroxidase (10 mg/ml, type II Sigma Chemical Co.)
Comments For typical endosome diameter of 250nm see Luby-Phelps 2000 PMID 10553280 p.191 top paragraph
Entered by Uri M
ID 104385