||Chinese hamster ovary (CHO)
||Sonawane ND, Szoka FC Jr, Verkman AS. Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes. J Biol Chem. 2003 Nov 7 278(45):44826-31.PubMed ID12944394
||This value is extracted from figure 4a. Researchers measured endosomal Cl- concentration, pH, and volume after internalization of polyplexes composed of plasmid DNA and polylysine (POL), a non-buffering polyamine, or the strongly buffering polyamines polyethylenimine (PEI) or polyamidoamine (PAM). [Cl-] and pH were measured by ratio imaging of fluorescently labeled polyplexes containing Cl- or pH indicators.
||The greater volume of polyplex-containing (macropinocytic) endosomes compared with endosomes formed by fluid-phase or receptor-mediated mechanisms permitted estimation of their volume by light microscopy. The averaged volume of fluorescent endosomes containing PAM polyplexes increased significantly with time after internalization, with a smaller increase for POL polyplexes. Polylysine (e-poly-L-lysine) is a small polypeptide of the essential amino acid L-lysine that is produced by bacterial fermentation.