Generation time on M9 glucose medium

Value 38 min Range: ±1.1 min
Organism Bacteria Escherichia coli
Reference Reshes G, Vanounou S, Fishov I, Feingold M. Timing the start of division in E. coli: a single-cell study. Phys Biol. 2008 Nov 75(4):46001 p.2 right column 3rd paragraphPubMed ID18997273
Method Abstract: "[Researchers] monitor the shape dynamics of individual E. coli cells using time-lapse microscopy together with accurate image analysis. This allows measuring the dynamics of single-cell parameters throughout the cell cycle."
Comments P.2 right column 3rd paragraph: "[Researchers] find that up to five generations, the growth of N is exponential. In LB, the average doubling time for the 30 cells was 18±0.5 min (BNID 104331) which is consistent with the generation time measured by monitoring OD600 of the corresponding liquid culture, 22 ± 0.6 min. The corresponding times for cells grown in M9-glucose were 38 ± 1.1 min and 48 ± 1.6 min, respectively. Although the difference between the generation times is significant (about 20% for both LB and M9-glucose), the faster growth rate on agar indicates that the solid support does not restrict cell growth."
Entered by Uri M
ID 104332