| Comments |
An examination of the accuracy of genome-wide expression datasets generated for Saccharomyces cerevisiae shows that the available datasets suffer from large random errors within studies as well as systematic shifts in reported values between studies, which make predictions of translational activity at the level of individual genes relatively inaccurate. In contrast, predictions of cell-wide translational activity are possible from such datasets with higher accuracy, and current datasets predict a production rate of about 13,000 proteins per haploid cell per second under fast growth conditions. This prediction is shown to be consistent with independently derived kinetic information on nucleotide exchange reactions that occur during translation, and on the ribosomal content of yeast cells. The predicted translation rates of 13,000 (6,500–19,500) proteins synthesised per second and of 6 (3–9) million peptide bonds (BNID 104314) being formed per second will form a useful benchmark against which emerging knowledge on the kinetics of relevant reactions can be interpreted. |