Radius of diffusion channel of nuclear pore complex NPC

Value 5.35 nm Range: 4.4-6.1 nm
Organism African clawed frog Xenopus laevis
Reference Keminer O, Peters R. Permeability of single nuclear pores. Biophys J. 1999 Jul77(1):217-28PubMed ID10388751
Method Optical single transporter recording (OSTR), for optically recording the activity of single transporters in membrane patches. Cells or organelles are firmly attached to isoporous filters, and the transport across membrane patches spanning filter pores is measured by confocal laser scanning microphotolysis (SCAMP) (Wedekind et al., 1994), the amalgamation of microphotolysis and laser scanning microscopy. By application of established theories for the restricted diffusion through small pores, the diffusion channel was approximated as a cylinder with a radius of 4.4-6.1 nm (mean 5.35 nm).
Comments See BNID 105654 for radius of 2.6 nm
Entered by Uri M
ID 104250