Time for DNMT1 to transfer a methyl group in vitro

Range 70-450 sec
Organism Human Homo sapiens
Reference Schermelleh L, Haemmer A, Spada F, Rösing N, Meilinger D, Rothbauer U, Cardoso MC, Leonhardt H. Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res. 200735(13):4301-12.PubMed ID17576694
Primary Source Pradhan S, Bacolla A, Wells RD, Roberts RJ. Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of de novo and maintenance methylation.J Biol Chem. 1999 Nov 12274(46):33002-10.PubMed ID10551868
Method A method is described to express and purify human DNA (cytosine-5) methyltransferase (human DNMT1) using a protein splicing (intein) fusion partner in a baculovirus expression vector.
Entered by Uri M
ID 104133