DNA replication rate of DNA polymerase II (slow-dna repair)

Range 20-30 nucleotides/sec
Organism Bacteria Escherichia coli
Reference Bonner CA, Stukenberg PT, Rajagopalan M, Eritja R, O'Donnell M, McEntee K, Echols H, Goodman MF. Processive DNA synthesis by DNA polymerase II mediated by DNA polymerase III accessory proteins. J Biol Chem. 1992 Jun 5267(16):11431-8.PubMed ID1534562
Method Research employed three methods to measure the size of newly synthesized DNA made after one association of the DNA polymerase with DNA primer termini: A biochemical kinetic approach, gel filtration, and detection of the product made from dnaG primase-primed G4 DNA in the presence of single-stranded DNA-binding protein.
Comments The intrinsic velocity of synthesis by pol II is 20-30 nucleotides per s with or without the accessory proteins.
Entered by Uri M
ID 104125