Processivity of Epstein-Barr virus DNA polymerase

Value 7200 nucleotides
Organism Epstein-Barr virus
Reference Tsurumi T. Primer terminus recognition and highly processive replication by Epstein-Barr virus DNA polymerase. Biochem J. 1991 Dec 15280 ( Pt 3):703-8PubMed ID1662485
Method EBV DNA polymerase was purified from B95-8 cells, which is a virus-producing marmoset lymphoblastoid cell line immortalized with human EBV from a mononucleosis patient, and treated with phorbol 12-myristate 13-acetate (PMA) and sodium n-butyrate as described in a previous report [ref 11].
Comments Competition experiments revealed that the EBV DNA polymerase had significantly higher affinity for primer termini hybridized to the template DNA than for the single-stranded DNA template or the single-stranded primer itself. ATP was not required either for primer terminus recognition or for sustainment of polymerization.
Entered by Uri M
ID 104117