Enzymes for Which kcat/Km Is Close to the Diffusion-Controlled Limit

Range Table - link
Organism Unspecified
Reference Nelson & Cox, Lehninger's Principles of Biochemistry 4th Edition, 2004, WH Freeman & Co, p.207, Table 6-8
Primary Source Fersht, A. (1999) Structure and Mechanism in Protein Science, p. 166, W. H. Freeman and Company, New York.
Comments "There is an upper limit to Kcat/Km, imposed by the rate at which E and S can diffuse together in an aqueous solution. This diffusion controlled limit is 10^8 to 10^9 M^-1×s^-1, and many enzymes have a kcat/Km near this range (table 6-8) Such enzymes are said to have achieved catalytic perfection. Note that different values of kcat and Km can produce the maximum ratio."
Entered by Uri M
ID 103917