Diffusion Coefficient of Bicoid morphogen in cytoplasm

Value 0.3 µm^2/sec Range: ±0.1 µm^2/sec
Organism Fruit fly Drosophila melanogaster
Reference Gregor T, Wieschaus EF, McGregor AP, Bialek W, Tank DW. Stability and nuclear dynamics of the bicoid morphogen gradient. Cell. 2007 Jul 13 130(1):141-52 DOI: 10.1016/j.cell.2007.05.026 p.147 left column 2nd paragraphPubMed ID17632061
Method P.147 left column 2nd paragraph: "To directly measure the diffusion constant of Bcd-GFP in the cortical cytoplasm, [investigators] measured the dynamics of recovery after pattern photobleaching. A rectangular volume 16 × 16 × 7 μm^3 in the anterior cytoplasm of Bcd-GFP-expressing embryos was photobleached during mitosis 13, when Bcd-GFP is uniformly distributed (locally) throughout the cortical cytoplasm and the cytoplasmic concentration is relatively high. A typical recovery curve is shown in Figure 5. Recovery curves were fit to solutions of the diffusion equation to estimate (see Experimental Procedures)."
Comments P.147 left column 2nd paragraph (continued from "Method" above): "From a total of 21 recovery curves in four embryos, [investigators'] analysis provided a cytoplasmic diffusion constant D = 0.30 ± 0.09 μm^2/s (mean ± SD) during mitosis 13, which is very close to [their] estimate from the transport model."
Entered by dandanbz
ID 103811