Number of actin molecules in cell

Value 1.43e+6 molecules per cell Range: ±240000 Table - link molecules per cell
Organism Fission yeast Schizosaccharomyces pombe
Reference Wu JQ, Pollard TD. Counting cytokinesis proteins globally and locally in fission yeast. Science. 2005 Oct 14 310(5746):pp. 312 Table 1PubMed ID16224022
Method Fusion yellow fluorescence protein (YFP)-cytokinesis protein measured by fluorescence or flow cytometry. Actin concentration measured by immunoblotting.
Comments Value is average of methods (A) and (B): A)Measurements of actin concentration by quantitative immunoblotting with internal standards and anti-actin antibody. B) Quantitation of cytoplasmic actin concentration (mean ± 1 standard deviation) of blots in (A) by Image J and densitometry. Assuming average cell volume of 92um^3.
Entered by Uri M
ID 103797