| Value |
13.5
Degrees
|
| Organism |
Generic |
| Reference |
Rivetti C, Walker C, Bustamante C. Polymer chain statistics and conformational analysis of DNA molecules with bends or sections of different flexibility. J Mol Biol. 1998 Jul 3280(1):41-59PubMed ID9653030
|
| Method |
A conformational analysis of double-stranded DNA containing bends or an intervening single-stranded region, provides a direct application of the equations presented above. In particular, the mean-square end-to-end distance, R2, was used first to quantify the bend angle of DNA molecules possessing a number of phased A-tracts. Second, the persistence length of a single-stranded DNA segment inserted between two double-stranded fragments was determined from the R2 of the entire polymer. |
| Comments |
First observed in kinetoplast DNA [Simpson 1979 and Challberg and Englund 1980], runs of four or more adenine residues (A-tracts) introduce intrinsic bends in DNA [Crothers et al 1990]. A-tract bending has been investigated using X-ray crystallography and other solution techniques, such as gel electrophoresis, electric birefringence, circular dichroism and NMR |
| Entered by |
Uri M |
| ID |
103795 |