Turnover rate of the glucose transporter ptsI (Subunit of enzyme II glc: PTS enzyme I)

Value 210 molecules/sec
Organism Bacteria Escherichia coli
Reference Waygood EB, Steeves T., Can J Biochem. 1980 Jan58(1):40-8. Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties. Table 1PubMed ID6992959
Method Preparation of Crude Extracts and Membranes, Assay of PTS Proteins and Enzymes, Sugar Phosphorylation Assay, Assays for HPr purification used methyl-alpha-[U-14C]-glucoside up to and including the Pellicon concentration, step. Polyacrylamide Gel Electrophoresis, Molecular Sieve Chromatography, Protein Determination, Sedimentation Velocity Studies,
Comments Equivalent to ~210 molecules/sec based on a MW of 135kD. Probably refers to the dimer state. Calculated from 0.000095 moles/min/mg (bion ). Multiply by 135000 Da and multiply by 1000mg/g, divide by 60 sec/min. 0.000095*135000*1000/60=213.75 molecules/sec. Calculated by Ron Milo
Entered by Uri M
ID 103693