| Value |
5
nmole/epicotyl
|
| Organism |
Azuki bean Vigna angularis |
| Reference |
Ooume K, Inoue Y, Soga K, Wakabayashi K, Fujii S, Yamamoto R, Hoson T. Cellular basis of growth suppression by submergence in azuki bean epicotyls. Ann Bot (Lond). 2009 Jan103(2):325-32.PubMed ID18940853
|
| Method |
ATP was extracted from frozen tissue powder with ice-cold 10 % perchloric acid (v/v) with 5 mM EDTA. The extract was centrifuged at 18 500 g for 10 min at 4 °C and the supernatant was collected. KOH at 3 M was then added to the supernatant to adjust the pH to 7–8. After the pH adjustment, the solution was centrifuged at 18 500 g for 10 min at 4 °C, and the supernatant was stored at –80 °C until assay. |
| Comments |
Seeds were submerged in water. Elongation rate and ATP content of the marked sub-hook region of 10 mm of Epicotyl was measured |
| Entered by |
Uri M |
| ID |
103666 |