Fraction of glutathione equivalents (GS) bound to protein (PSSG) in ER of liver

Value >=50 %
Organism Rat Rattus norvegicus
Reference Hansen RE, Roth D, Winther JR. Quantifying the global cellular thiol-disulfide status. Proc Natl Acad Sci U S A. 2009 Jan 13106(2):422-7.PubMed ID19122143
Primary Source Bass R, Ruddock LW, Klappa P, Freedman RB.A major fraction of endoplasmic reticulum-located glutathione is present as mixed disulfides with protein. J Biol Chem. 2004 Feb 13279(7):5257-62.PubMed ID14630926
Method The distribution of glutathione between different chemical states in rat liver microsomes was determined by labeling with the thiol-specific label monobromobimane and subsequent separation by reversed phase high performance liquid chromatography.
Entered by Uri M
ID 103541