Fraction of misincorporation of aa-tRNA occuring during initial selection stage

Value ~20 %
Organism Bacteria Escherichia coli
Reference Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009, Pages 746-762PubMed ID19239893
Primary Source Blanchard SC, Gonzalez RL, Kim HD, Chu S, Puglisi JD. tRNA selection and kinetic proofreading in translation. Nat Struct Mol Biol. 2004 Oct11(10):1008-14.PubMed ID15448679
Method Single-molecule fluorescence resonance energy transfer (smFRET). Intermediate states in the pathway of tRNA delivery were observed using antibiotics and nonhydrolyzable GTP analogs. Measured under relatively high magnesium, low fidelity conditions
Comments Initial selection is comprised of: Initial binding, coding recognition and GTPase activation
Entered by Uri M
ID 103485