Specific activity of polyphosphate-ADP-phosphotransferase

Value 0.2 (nmol P)*min^-1*(mg protein)^-1
Organism Amoeba Dictyostelium discoideum
Reference Gezelius K. Inorganic polyphosphates and enzymes of polyphosphate metabolism in the cellular slime mold Dictyostelium discoideum. Arch Microbiol. 1974 Jul 2298(4):311-29.PubMed ID4367840
Method Extraction and Assay of Polyphosphate-ADP-Phosphotransferase. Cell-free extracts were obtained through centrifugation at 3000 g for 15 min. When tested at the culmination and sorocarp stages, ca 95% of the total activity was recovered in the supernatant.The enzyme activities were assayed at 23 degrees C in the direction of ATP
Comments P in units stands for polyphosphates. The specific activity of the polyphosphate-ADP-phosphotransferase, pH optimum 7.7, was ca 0.2 nmol Pi·min-1·mg protein-1 and rather constant during differentiation. Reaction investigated was P(n-1)+ATP>>Pn+ADP
Entered by Uri M
ID 103424