Km of PolyA polymerase

Value 0.308 mM Range: ±0.12 mM
Organism Budding yeast Saccharomyces cerevisiae
Reference Sillero MA, De Diego A, Osorio H, Sillero A. Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase. Eur J Biochem. 2002 Nov269(21):5323-9.PubMed ID12392566
Method Poly(A) polymerase from yeast was prepared. One unit of enzyme is the amount that incorporates 1 nmol of ATP (as AMP) into an acid insoluble form in 1 min at 37 °C. After incubation at 30 degrees or 37 degrees, the reaction mixtures were analyzed by TLC or HPLC
Comments Km calculated for S[0.5] (substrate at half of Vmax)
Entered by Uri M
ID 103418