Km for activation of Pyruvate Pi dikinase by Pi

Value 650 uM
Organism Corn Zea mays
Reference Burnell JN, Hatch MD. Regulation of C4 photosynthesis: purification and properties of the protein catalyzing ADP-mediated inactivation and Pi-mediated activation of pyruvate,Pi dikinase. Arch Biochem Biophys. 1985 Mar237(2):490-503.PubMed ID2983615
Method Maize plants (Zea mays cv. Dekalb XL81) were grown in soil in a glasshouse between 12 and 30°C leaf tissue was homogenized to extract PDRP. Assay of activation and inactivation of pyruvate,Pi dikinase. All assays were conducted at pH 8.3 unless stated otherwise.
Comments This value for the reaction: PDRP(with thr and his residues)+ATP+PI=E(with thr residue and phosphorylated His residue) + AMP +PPi
Entered by Uri M
ID 103411