Ratio of adenylate kinase concentration between C4 plants/C3 plants
Value | >=30 Unitless |
---|---|
Organism | Plants |
Reference | Hatch M. D., Properties and Regulation of Adenylate Kinase from Zea mays Leaf Operating in C4 Pathway Photosynthesis, Aust. J. Plant Physiol., 1982, 9, 287-96 |
Method | Approximately 200 g of maize leaves were extracted by blending for 90 s in a Waring blendor with 1500 mL of ice-cold medium containing 25 mM HEPES-KOH buffer, pH 7.2, 5 mM MgCl2, 50 mM 2-mercaptoethanol and 1 mM EDTA. This procedure extracts mesophyll cells but leaves the majority of bundle sheath cells intact, centrifugation and separation of adenylate kinase fraction |
Entered by | Uri M |
ID | 103404 |