Amount of potassium pumped through sodium-potassium pump in normal Krebs solution

Value 0.444 mmole/kg fresh weight/min
Organism Guinea Pig Cavia porcellus
Reference Brading AF, Widdicombe JH. An estimate of sodium-potassium pump activity and the number of pump sites in the smooth muscle of the guinea-pig taenia coli, using (3H)ouabain. J Physiol. 1974 Apr238(2):235-49. abstractPubMed ID4840873
Method Animals killed, caecum dissected, put in Krebs solution. They were then blotted and weighed, and after washing with ouabain/42K solution counted in a gamma scintillation counter, to determine the uptake of potassium by the tissue.
Comments Abstract: "4. The amount of potassium pumped into the cells at various concentrations of extracellular potassium has been calculated. In normal Krebs solution the amount pumped in 45 min was 20.0 m-mole/kg fresh wt., and this was increased at higher potassium concentrations." This is an average value arrived at by dividing the value for 45 min (20 mmole/kg fresh tissue). The value increases when potassium levels are increased.
Entered by Uri M
ID 103032