||Boireau S, Maiuri P, Basyuk E, de la Mata M, Knezevich A, Pradet-Balade B, Bäcker V, Kornblihtt A, Marcello A, Bertrand E. The transcriptional cycle of HIV-1 in real-time and live cells.J Cell Biol. 2007 Oct 22 179(2):291-304.PubMed ID17954611
||Using human immunodeficiency virus (HIV) type 1 reporters, real-time messenger RNA (mRNA) biogenesis was studied by photobleaching nascent RNAs and RNAPII at specific transcription sites. Through modeling, the use of mutant polymerases, drugs, and quantitative in situ hybridization, the kinetics of the HIV-1 transcription cycle was investigated. In human U2OS osteosarcoma cells.
||In vitro kinetic analyses of human RNAPII have yielded elongation rates between 0.9 and 1.8 kb/min and have suggested that the chemical step is rate limiting (Burton et al., 2005). Researchers' in vivo data are in the upper range of these values, indicating that RNAPII may work close to its maximal speed.