Percent of rRNA and tRNA from total RNA

Range 95–97 %
Organism bacteria
Reference Rosenow, C., et al., Prokaryotic RNA preparation methods useful for high density array analysis: comparison of two approaches. Nucleic Acids Res, 2001. 29(22): p.1 right column top paragraphPubMed ID11713332
Comments P.1 right column top paragraph: "Thus, labeling of bacterial RNAs requires either direct labeling of total RNA or the use of a cDNA synthesis process in which a pool of gene-specific or random oligonucleotide primers replace the oligo(dT) primers (refs 9,13). Neither sample preparation method enriches the labeled target for mRNA. Thus, much of the label is incorporated into rRNAs and tRNAs, which make up 95–97% of the total RNA isolated from bacterial cells." See Sørensen et al., 2017 PMID 29023189 p.6 top paragraph: "Since tRNA and rRNA together make up more than 95% of the total RNA, (refs 26, 27, in E. coli)..."
Entered by omri
ID 102939