Turnover rate of the glucose transporter ptsI (Subunit of enzyme II glc: PTS enzyme I)

Value 95 µmol/min/mg Range: (≈210 molecules/sec) µmol/min/mg
Organism Bacteria Escherichia coli
Reference BRENDA (BRaunschweig ENzyme DAtabase) link
Primary Source Waygood EB, Steeves T., Can J Biochem. 1980 Jan58(1):40-8. Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties. p.44 Table 1PubMed ID6992959
Method Primary source p. 41 right column 3rd paragraph: "The assay procedures used were essentially the same as have been recently described (ref 4 PMID 386829), with some modifications. There are two types of assays used: the sugar phosphorylation assay and the lactate dehydrogenase coupled assay, both of which are given below."
Comments Equivalent to ~210 molecules/sec based on a MW of 135kDa. Probably refers to the dimer state.
Entered by Ron Milo - Admin
ID 102931