Virtual mitochondrial number per cell

Range 83-677 virtual mitochondria/cell
Organism Mammals
Reference Robin ED, Wong R. Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells. J Cell Physiol. 1988 Sep136(3):507-13. abstract & p.510 table 2PubMed ID3170646
Method P.509 left column 3rd paragraph: "Mitochondria were isolated from cell suspensions according to the "no gradient" method of Bogenhagen and Clayton (Bogenhagen and Clayton, 1974)." P.509 left column bottom paragraph: "Quantitation of mtDNA: Mitochondrial DNA (mt DNA) was measured using the method of Tapper and Clayton (Murphy et al., 1984)." P.509 right column 2nd paragraph: "Mitochondrial counting: Isolated mitochondria from all cell types were stained with Rhodamine 123 according to the method of Johnson, Walsh, and Chen (Johnson et al., 1980)." P.509 right column 3rd paragraph: "Enzyme assays: Cytochrome aa3 was measured spectrophotometrically by the method of Simon et al. (1973)." P.509 right column 4th paragraph: "Malate dehydrogenase: Malate dehydrogenase was measured spectrophotometrically by the method of Rudolph, Baugher, and Beissner (Rudolph et al., 1977)." Using the Cyaa3 method
Comments Abstract: "Virtual mt number/cell ranged from 83±17 to 677±80 (SE) mt/cell in various cell types." P.508 left column 5th paragraph: "The term “virtual” was used to encompass the possibility that each cell possesses one giant individual mitochondrion and that the number derived by this method refers to mitochondrial fragments resulting from mitochondrial isolation procedures." P.511 left column 3rd paragraph: "In contrast to the constancy of mt DNA/mt, virtual mt number/cell varied widely from cell type to cell type as noted above, ranging from 88±12 (SE) in rabbit peritoneal macrophages to 688±75 in rabbit lung macrophages. Within any single cell type, the variation is small (less than 10%)."
Entered by Uri M
ID 102920