Number of neurotransmitter molecules in typical synaptic vesicle (at 150 mM)

Value 1790 copies/vesicle
Organism Rat Rattus norvegicus
Reference Takamori S. et al., Molecular anatomy of a trafficking organelle. Cell. 2006 Nov 17 127(4):831-46. p.839 table 2 & p.843 right column 2nd paragraphPubMed ID17110340
Method P.832 left column 3rd paragraph: "...[Researchers] used a purified SV [synaptic vesicle] fraction from rat brain as the starting point for the description of a prototypic SV, resulting in a detailed (albeit averaged) structural model of this trafficking organelle."
Comments P.843 right column 2nd paragraph: "SVs contain neurotransmitter transporters that are fueled by a proton electrochemical gradient (reviewed in Ahnert-Hilger et al., 2003). [Researchers'] results show that vesicles contain approximately ten copies of either VGLUT1 or VGLUT2, and it is likely that the copy number for VGAT, VAChT, or VMAT1/2 is similar on the respective vesicles. With a membrane thickness of 4 nm, the inner volume of an average vesicle is 19.86×10^-21l (BNID 102776), equal to 1790 molecules of glutamate assuming a concentration of 150 mM (Table 2)."
Entered by Uri M
ID 102777