Ratio between CA I to CA II binding to dansylamide
Value | 100 Fold |
---|---|
Organism | Human Homo sapiens |
Reference | Lindskog S. Structure and mechanism of carbonic anhydrase. Pharmacol Ther. 199774(1):pp. 2PubMed ID9336012 |
Primary Source | Engstrand C, Jonsson BH, Lindskog S. Catalytic and inhibitor-binding properties of some active-site mutants of human carbonic anhydrase I. Eur J Biochem. 1995 May 1229(3):696-702.PubMed ID7758465 |
Method | Three isozyme-specific residues in the active site of human carbonic anhydrase I, Val62, His67, and His200, have been changed by site-directed mutagenesis to their counterparts in human carbonic anhydrase II, Asn62, Asn67, and Thr200 |
Comments | Dansylamide binds 100 times more strongly to human CA I than to CA II |
Entered by | Uri M |
ID | 102671 |