Ratio between CA I to CA II binding to dansylamide

Value 100 Fold
Organism Human Homo sapiens
Reference Lindskog S. Structure and mechanism of carbonic anhydrase. Pharmacol Ther. 199774(1):pp. 2PubMed ID9336012
Primary Source Engstrand C, Jonsson BH, Lindskog S. Catalytic and inhibitor-binding properties of some active-site mutants of human carbonic anhydrase I. Eur J Biochem. 1995 May 1229(3):696-702.PubMed ID7758465
Method Three isozyme-specific residues in the active site of human carbonic anhydrase I, Val62, His67, and His200, have been changed by site-directed mutagenesis to their counterparts in human carbonic anhydrase II, Asn62, Asn67, and Thr200
Comments Dansylamide binds 100 times more strongly to human CA I than to CA II
Entered by Uri M
ID 102671