Carbonic Anhydrase III CO2 hydration turnover rate

Value <=8000 1/sec
Organism Human Homo sapiens
Reference Lindskog S. Structure and mechanism of carbonic anhydrase. Pharmacol Ther. 1997 74(1):1-20.PubMed ID9336012
Primary Source Jewell DA, Tu CK, Paranawithana SR, Tanhauser SM, LoGrasso PV, Laipis PJ, Silverman DN. Enhancement of the catalytic properties of human carbonic anhydrase III by site-directed mutagenesis.Biochemistry. 1991 Feb 12 30(6):1484-90PubMed ID1899618
Method Catalysis by wild-type HCA III and mutants was determined from the initial velocity of hydration of CO2 at steady state by stopped-flow spectrophotometry and from the exchange of 18O between CO2 and water at chemical equilibrium by mass spectrometry.
Comments at 25°C
Entered by Uri M
ID 102665