Decrease in Kmax of fdh for when cysteine replaces selenocysteine

Value ~3 Fold
Organism Bacteria Escherichia coli
Reference Axley MJ, Böck A, Stadtman TC. Catalytic properties of an Escherichia coli formate dehydrogenase mutant in which sulfur replaces selenium. Proc Natl Acad Sci U S A. 1991 Oct 188(19):pp. 8453 table 1, fig. 4(B)PubMed ID1924303
Method Enzyme activity was determined as a function of formate and BV (benzyl viologen) concentrations.
Comments Kmax of original fdh with selenocysteine is 26 mM. In mutant (s)fdh with cysteine replacing selenocysteine kmax=9 mM.
Entered by Uri M
ID 102622