Percent of selenocysteine incorporated to protein after being mutagenized to code cysteine

Value 10 %
Organism Bacteria Escherichia coli
Reference Zinoni F, Birkmann A, Leinfelder W, Böck A. Cotranslational insertion of selenocysteine into formate dehydrogenase from Escherichia coli directed by a UGA codon. Proc Natl Acad Sci U S A. 1987 May84(10):3156-60.PubMed ID3033637
Method By localized mutagenesis, the UGA codon was converted into serine (UCA) and cysteine (UGC and UGU) codons. Each mutation relieved the selenium dependency of fdhF mRNA translation.
Comments in-frame UGA codon at amino acid position 140 that is translated to selenocysteine. Alteration of codon to code cysteine abolished selenocysteine incorporation
Entered by Uri M
ID 102614