Excited GFP lifetime in cell cytoplasm

Value 2.6 nanosecond
Organism Chinese hamster ovary (CHO)
Reference Swaminathan R, Hoang CP, Verkman AS. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys J. 1997 Apr72(4):1900-7.PubMed ID9083693
Method GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm.
Entered by Uri M
ID 102559