Half time for reversible photobleaching of GFP

Range 1.5-5.5 ms
Organism Chinese hamster ovary (CHO)
Reference Swaminathan R, Hoang CP, Verkman AS. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys J. 1997 Apr72(4):1900-7.PubMed ID9083693
Method GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm.
Comments relative viscosity 5-250. This is half of the time of photobleaching that allows recovery of fluorescence
Entered by Uri M
ID 102558