Oxidized glutathione GSSG concentration in blood

Range 2-6 uM
Organism Human Homo sapiens
Reference Rossi R, Blood glutathione disulfide: in vivo factor or in vitro artifact? Clin Chem. 2002 May48(5):742-53.PubMed ID11978601
Method Researcehers measured GSH and GSSG using a spectrophotometer with a modification of the GSH recycling method the same samples were also measured by reversed-phase HPLC after derivatization of thiols (dithiothreitol was used to reduce disulfides) with monobromobimane. The thiol-bimane adduct was measured by a fluorescence detector.
Comments GSSG is present in healthy human blood at low concentrations (2-6 micromol/L), and most published data on GSSG may be affected by artifacts, causes of several artifacts are listed in paper. For blood concentration of GSH please see BNID 102529
Entered by Uri M
ID 102530