Fraction of isotonic cell volume that is osmotically inactive

Value 17.8 %
Organism Pig Sus species
Reference Mullen SF, Rosenbaum M, Critser JK. The effect of osmotic stress on the cell volume, metaphase II spindle and developmental potential of in vitro matured porcine oocytes. Cryobiology. 2007 Jun54(3):281-9PubMed ID17485076
Method Extrapolation of the relationship between equilibrated cell volume and solution concentration Y=0.824X+0.178 to infinite osmolality suggests that the osmotically inactive cell volume (Vb) is 17.8% of the isotonic volume. Oocytes were incubated for 10 min at 38 degrees C in various hypo- or hypertonic solutions, and an isotonic control solution and then assessed for these two parameters
Comments Oocyte volume displayed a linear response to anisotonic solutions as expected
Entered by Uri M
ID 102426