Extinction coefficient of mYFP

Value 79000 1/(M*cm)
Organism Fission yeast Schizosaccharomyces pombe
Reference Wu JQ, Pollard TD. Counting cytokinesis proteins globally and locally in fission yeast. Science. 2005 Oct 14 310(5746): Supporting Online Material for reference at www.sciencemag.org/cgi/content/full/310/5746/310/DC1 p.4 2nd paragraphPubMed ID16224022
Primary Source Zacharias DA, Violin JD, Newton AC, Tsien RY. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science. 2002 May 3 296(5569):913-6.PubMed ID11988576
Method "The mYFP concentration was determined by absorbance at 514 nm using an extinction coefficient of 79,000 M-1 cm-1 (primary source)."
Entered by Uri M
ID 102276