| Value |
1000
Fold
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Benno Muller-hill, 1996, The Lac Operon: A Short History Of A Genetic Paradigm, Publisher: Walter de Gruyter. Berlin. New York. Part 3.2 pp.134 |
| Primary Source |
Schlax PJ, Capp MW, Record MT Jr.,Inhibition of transcription initiation by lac repressor, J Mol Biol. 1995 Jan 27245(4):331-50PubMed ID7837267
|
| Method |
Effects of lac repressor on the extent of formation and the kinetics of association and dissociation of abortively-initiating open complexes (RPinit) were examined using fluorescence detected abortive initiation and KMnO4 chemical probing. |
| Comments |
Lac repressor bound to a suitably placed lac operator sterically inhibits functional binding (open complex formation) of RNA pol to its promoter in its activated state 1000 fold |
| Entered by |
Uri M |
| ID |
102075 |