Chloramphenicol acetyltransferase (cat) specific activity in cointegrate

Value 263.4 nmol acetylated chloramphenicol formed/min/mg protein Range: ±28.2 nmol acetylated chloramphenicol formed/min/mg protein
Organism Bacteria Escherichia coli
Reference Li SJ, Cronan JE Jr., Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis.J Bacteriol. 1993 Jan175(2):pp. 338 Figure 4- average of five cointegrates from topPubMed ID7678242
Method Strains carrying promoter fusion plasmids or F' cointegrated derivatives were grown to the mid-log phase in RB media containing appropriate antibiotics for plasmid maintenance.
Comments CAT specific activity is indicator of accBC operon expression. A cointegrate is the intermediate molecule which donor DNA and target DNA covalently bind during the formation of a Holliday junction.
Entered by Uri M
ID 102062