mRNA degradation constant for a fusion protein (tsr-Venus YFP)

Value 1.5 min Range: ±0.2 min
Organism Bacteria Escherichia coli
Reference X. Sunney Xie, Paul J. Choi, Gene-Wei Li, Nam Ki Lee, and Giuseppe Lia, 'Single-Molecule Approach to Molecular Biology in Living Bacterial Cells', Annu. Rev. Biophys. 2008. 37:p.433PubMed ID18573089
Primary Source Yu J, Xiao J, Ren X, Lao K, Xie XS. 2006. Probing gene expression in cells, one molecule at a time. Science 311:1600–3PubMed ID16543458
Method After a short period of induction, the mRNA production is inhibited by an antibiotic and the amount of tsr-venus mRNA is measured using real-time PCR.
Comments TSR=thrombospondin structural homology repeat, a highly structured domain organized as a long right-handed spiraling ribbon containing three antiparallel strands
Entered by Uri M
ID 102045